• Stable lentivector-based system
• Ideal for co-localization studies
• Monitor protein trafficking in real-time
• Organelles, vesicles and compartments
Vivid tracking of subcellular activities made simple with Cyto-Tracers™
Molecular trafficking is a dynamic process in eukaryotic cells and the Cyto-Tracers™ provide the ability to light up cell compartments to monitor movement and localization of organelles and to trace endocytosis and exocytosis. SBI has created a line of lentivector-based Cyto-Tracers™ that utilize GFP-fusion proteins to mark cellular compartments, organelles, vesicles and structures to enable more long-term and more in-depth experimentation. The Cyto-Tracers can be used intransfections as well as packaged into virus to create stable GFP tracer cell lines in primary cells, tumor cell lines and stem cells.
Live-cell tracing for dynamic in vitro and in vivo studies
List of pre-made Cyto-Tracers
Cyto-Tracer
Target Location
Peptide Tag
pCT-Mem-GFP
Plasma Membrane
Neuromodulin
pCT-InnerMem-GFP
Inner Leaflet of Membrane
Farnesylation signal
pCT-Mito-GFP
Mitochondria
Cytochrome C oxidase VIII
pCT-ER-GFP
Endoplasmic Reticulum
ER targeting consensus
pCT-Golgi-GFP
Golgi Apparatus
Galactosyltrasferase
pCT-Secretory-GFP
ER-Golgi Vesicles
Secretory consensus peptide
pCT-Lyso-GFP
Lysosome
Cathepsin B
pCT-Endo-GFP
Endosome
RhoB
pCT-Pero-GFP
Peroxisome
Peroxisomal consensus signal
pCT-Autophago-GFP
Autophagosome
LC3
pCT-CD63-GFP
Exosome/Secretory
CD63 Tetraspanin
pCT-Actin-GFP
Cytoskeleton
β-actin
pCT-Tublin-GFP
Cytoskeleton
α-tublin
pCT-MAP4-GFP
Microtubule
Microtubule associated protein 4 (MAP4)
pCT-H2B-GFP
Nucleus
H2B
pCT-Apoptosis-Luc
Caspase 3/7 activation
Cyclic inactive luciferase
pCT-GFP-BAX
Cytosol to Mitochondria
BAX
pCT- Catenin-GFP
Cytosol to Nucleus/Cell Membrane
β-Catenin activation
pCT-CMV-PSD95-GFP
Dendrite Membranes
PSD-95
pCT-Cyto-GFP
Cytosolic Compartment
GFP-Untagged
pCT-Cyto-RFP
Cytosolic Compartment
RFP-Untagged
pCT-Cyto-GFpLuc
GFP and Luciferase Control
GFP and Luciferase (Untagged)
Trace Exosomes with CD63-GFP fusion Cyto-Tracers
The Tetraspanin CD63 protein is a common biomarker for exosomes. With the pCT-CD63-GFP construct you can make you cells of interest secrete exosomes that glow green for downstream functional delivery studies (catalog# CYTO120-PA-1).
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