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High level of expression from CMV promoter
The multiple cloning site (MCS) located
downstream of a CMV promoter allows for convenient cloning of your
gene of interest. The SV40 polyadenylation signal in both vectors
enables efficient termination of transcription, resulting in high
levels of steady-state mRNA.
Reliable delivery to dividing or non-dividing cells
When used with lentiviral packaging
plasmids, your cDNA constructs can be packaged into VSV-G
pseudotyped viral particles and delivered into a wide range of
dividing and quiescent mammalian cells—including
difficult-to-transfect cells such as primary cultures, stem cells,
neurons, endothelial cells, and retinoblastomas—with high
efficiency. The result is a stable cell line that expresses the
gene of interest. The system can also be used to overexpress genes
in model organisms.
Convenient selection of transfected cells
In addition to the pCD one-promoter
vectors, which carry the multiple cloning site downstream of the CMV promoter, the pCD system also offers the option of a second
expression cassette downstream of the MCS to express the puromycin
resistance gene or copGFP as a reporter under the control of a
constitutive human elongation factor 1 (EF1) promoter which is
functional in most cell lines.
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