RNAi Cloning and Expression Vector List
FIV-Based Single-Promoter pSIF1-H1-Puro shRNA Vector
Cat. # SI100C-1, 10 mg
· Express short-hairpin shRNA from the H1 RNA polymerase III promoter
· Package expression construct into lentiviral pseudoviral particles and deliver to nearly any mammalian cell
· Select stable knockouts using puromycin resistance
· FIV (feline immunodeficiency virus) based, replication-incompetent design
User manual • Sequence (text) • Price list
FIV-Based Single-Promoter pSIF1-H1-copGFP shRNA Vector
Cat. # SI101B-1, 10 mg
· Select stable knockouts using a fluorescent copepod GFP (copGFP) marker
HIV-Based Single-Promoter pSIH1-H1-Puro shRNA Vector
Cat. # SI500A-1, 10 mg
· Select stable knockouts using a fluorescent copepod GFP marker
· HIV (human immunodeficiency virus) based, replication-incompetent design
HIV-Based Single-Promoter pSIH1-H1-copGFP shRNA Vector
Cat. # SI501A-1, 10 mg
· HIV (human immunodeficiency virus) based, replication-incompetent
HIV-Based Single-Promoter pSIH1-H1-H2Kk shRNA Vector
Cat. # SI502A-1, 10 mg
· Select stable knockouts using H2Kk-FITC antibodies
FIV-Based Double-Promoter pFIV-H1/U6-Puro siRNA Vector
Cat. # SI110A-1, 10 mg
· Express double-stranded siRNA template sequences from the opposing H1 and U6 RNA polymerase III promoters
· Package expression construct into lentiviral pseudoviral particles and deliver to any mammalian cell
FIV-Based Double-Promoter pFIV-H1/U6-copGFP siRNA Vector
Cat. # SI111A-1, 10 mg
· Deliver siRNA expression constructs to any cell by lentiviral transduction
RNAi Lentivector Kit Components
Each Vector Kit includes 10 mg of plasmid DNA, a ligation control, PCR primers to identify positive clones, and a complete protocol.
Note: All functional elements except for the AmpR region of these vectors have been completely sequenced.
Discontinued pFIV-H1 Vector Sequences
pFIV-H1-Puro (Cat.# SI100B-1) pFIV-H1-copGFP (Cat.# SI101A-1)
