Features and Benefits

• Faster than conventional p24 ELISA titer kits (less than 3 hours from cells-to-titer!)

• Accurate qPCR measurement of lentiviral titers.

• No isolation or concentration of genomic DNA required.

• Built-in reference control for normalization.

• Dynamic titering up to 15 MOI.

• Compatible with most WPRE-containing lenti-constructs. (ex. pLVCT-H1, pLVTHM, pGIPz, HiPerform™ and all SBI lentivectors)

Product Overview
Kit Components
User Manual
Ordering Information
Related Products

Product Overview

The UltraRapid Lentiviral Titer Kit is designed to rapidly determine the titers of pseudoviral particles that are generated with SBI’s FIV and HIV lentiviral vectors or libraries. It allows users to measure the copy numbers of integrated lentiviral constructs in genomic DNA of transduced target cells. Except for nuclease-free water, the kit contains all components necessary for measuring the amounts of endogenous PGK1 DNA and the pseudo-lentiviral-specific WPRE element that is integrated into the genomes of successfully tranduced cells in each sample. The titer of each sample is then determined by calculating the amount of WPRE element relative to that of PGK1 DNA against a standard curve generated with the provided calibration standards.

Flow chart of the Experimental Procedure:

Example of a Real-time PCR setup
 

Sample Data

Sample A: amplification plot of PGK1 genomic DNA of calibration standards

Sample B: dissociation curves of the amplified PGK1

Sample C: amplification plot of WPRE DNA of the calibration standards

Sample D: dissociation curves of the amplified WPRE

Sample E: standard curve generated with the Ct data obtained from the experiment shown in Panels A and C

Sample F: amplification plot of a sample experiment

Kit Components

• Cell lysis buffer

• Primer sets

• Calibration standards

• 2x SYBR Green hotstart Taq DNA polymerase master mix

• User Manual

• Product Analysis Certification (PAC) Sheet
   

Shipped on dry ice, should be stored at -20°C upon receipt

User Manual

PDF User Manual 

Ordering Information