Packaged Positive Transduction Control

  • Easily visualize and quantify transduction efficiency with copGFP fluorescence
  • Optimize transduction conditions for your cell system
  • Test the sensitivity of target cells to lentiviral transduction with SBI's FIV or HIV-based systems

CrFK cells transduced with the
pSIF1-H1-siLuc-copGFP Transduction Control.

 

The Packaged Positive Transduction Control can be directly used to optimize transduction conditions or determine the sensitivity of target cells to infection with packaged lentivector constructs. Pre-packaged in VSV-G pseudotyped viral particles, the Packaged Transduction Control is provided in a ready-to-use format that allows you to immediately transduce your cells on receipt. Transduced cells stably express the copepod green fluorescent protein (GFP) marker. You can easily detect cells that express the copGFP gene by fluorescent microscopy or by fluorescence-activated cell sorting (FACS). This control can be used to optimize the protocol for delivery of pFIV constructs into your target cells in order to determine the necessary transduction efficiency.

 

Positive Transduction Controls

Origin

Size

Catalog #

Manual

pSIF1-H1-siLuc-copGFP Packaged Control

FIV

2 x 105 ifu

LV201B-1

PDF

pSIH1-H1-siLuc-copGFP Packaged Control

HIV

2 x 105 ifu

LV601B-1

PDF

Price List

 

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