High-throughput MicroRNA Profiling Using Powerful and Sensitive Real-time qPCR

Features and Benefits

• Simultaneously profile 95 different MicroRNAs known to be involved in Stem Cell self-renewal, hematopoiesis, neural development and tissue patterning

• Identify MicroRNA development biomarkers and expression pattern signatures in Stem Cells

• System rapidly tags and converts all small RNAs into detectable cDNA for qPCR

• Simple and robust procedure

• Sensitive and quantitative down to picogram amounts of starting total RNA

• Amenable to high-throughput screening of numerous cell lines and tissues

The Stem Cells microRNA Panel monitors Key Differentiation pathways
The microRNAs in the Stem Cell Assay Panel Address:

• Self-renewal

• Hematopoiesis-specific

• Neural-Specific

• Differentiation-Specific

Product Overview
References
Kit Components
User Manual
Ordering Information
Related Products

Stem Cells MicroRNA qPCR Panel Overview

What this Array Kit does:

The new Stem Cells MicroRNA qPCR Array enables the simultaneous quantitation of 95 microRNAs known to be involved in Stem Cell biology.

The technology utilizes the QuantiMir RT Kit which tags and converts all small RNAs into cDNA, ready to use as template for real-time qPCR.
 

• Quantitate and profile MicroRNA expression levels from any cell type or tissue source

• Discover Biomarkers in Stem Cell development

• Identify expression pattern signatures in Stem Cell lineages

• Entire procedure takes 1 day
   

	No more messy Northerns or RNase protection assays! Say good-bye to Radioactivity.
	Stop wasting your precious RNA on a single MicroRNA detection per RT reaction!
	Profile 95 different MicroRNAs simultaneously from 1 RT reaction!

SBI has tested and recommends SYBR Green Master mix from three vendors: Power SYBR Master Mix® (Cat numbers 4368577, 4367650, 4367659, 4368706, 4368702, 4368708, 4367660) from Applied Biosystems; SYBR GreenER™ qPCR SuperMix for ABI PRISM® instrument from Invitrogen (Cat numbers 11760-100, 11760-500, and 11760-02K); and RT² Real-Time™ SYBR Green / ROX PCR (Cat numbers PA-012 and PA-112) from SuperArray. For details of the buffer recommendation and experimental conditions, please see User Manual.
 

Stem Cells MicroRNA qPCR Panel Layout

click to enlarge

Sample MicroRNA qPCR Panel Data:
The Stem Cells microRNA qPCR Panel was used to quantitate microRNAs in Human Cord Blood CD34+ Stem Cells.

Real-time amplification plot

 

click to enlarge

Graphical representation of relative abundance of microRNAs in Human Cord Blood CD34+ Stem Cells (levels Normalized to U6)

Technical References
Shi, R. and Chiang, V.L. 2005. Facile means for quantifying microRNA expression by real-time PCR. BioTechniques. 39:519-525.

Fortner, D.M., Troy, R.G., and Brow D.A. 1994. A stem/loop in U6 RNA defines a conformational switch required for pre-mRNA splicing. Genes and Development. 8(2): 221-233.

Stem Cell and MicroRNA References

• DGCR8 is essential for microRNA biogenesis and silencing of embryonic stem cell self-renewal.
  Nat Genet. 2007 Mar;39(3):380-5.
  Wang Y, Medvid R, Melton C, Jaenisch R, Blelloch R.

• Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing.
  Genes Dev. 2005 Feb 15;19(4):489-501.
  Kanellopoulou C, Muljo SA, Kung AL, Ganesan S, Drapkin R, Jenuwein T, Livingston DM, Rajewsky K.

• MicroRNA expression profiling during human cord blood-derived CD34 cell erythropoiesis.
  Exp Hematol. 2007 Apr;35(4):551-64.
  Choong ML, Yang HH, McNiece I.

• MicroRNA expression profiling of single whole embryonic stem cells.
  Nucleic Acids Res. 2006 Jan 24;34(2):e9.
  Tang F, Hajkova P, Barton SC, Lao K, Surani MA.

• Stem cells: from epigenetics to microRNAs.
  Neuron. 2005 May 5;46(3):363-7. Review.
  Cheng LC, Tavazoie M, Doetsch F.

• MicroRNA: a new player in stem cells.
  J Cell Physiol. 2006 Nov;209(2):266-9. Review.
  Zhang B, Pan X, Anderson TA.

• Human embryonic stem cells express a unique set of microRNAs.
  Dev Biol. 2004 Jun 15;270(2):488-98.
  Suh MR, Lee Y, Kim JY, Kim SK, Moon SH, Lee JY, Cha KY, Chung HM, Yoon HS, Moon SY, Kim VN, Kim KS.
   

Kit Components

+ One 96-well Stem Cells microRNA Primer stock plate (enough for 10 entire panel measurements)

Kits should be stored at -20^oC upon receipt.


User Manual

Ordering Information

To obtain more information contact SBI at 888-266-5066 (toll-free in North America) or 650-968-2200, or e-mail to orders@systembio.com

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