SBI is
offering a new product class to study stem cell differentiation:
Stem Cell Reporters
in Lentivectors or Pre-packaged Particles
Features and Benefits
-
Lentiviral-based reporters for studying cell
differentiation, especially applied to stem cells
-
Cell-specific promoters drive GFP and Zeocin selection in
differentiated cells—monitor differentiation in real-time
-
Isolate pure populations of differentiated cells by GFP
sorting or by Zeocin selection
-
Rapidly create transgenic lines and ES reporter cells
-
Generate stable reporter lines for high-throughput
screening—can be combined with SBI’s genome-wide shRNA
libraries
-
Various different cell lineages can be studied
(Table of
Available Reporters)
Product Overview
Kit Components
List of
Differentiation Reporters
User Manual
Ordering Information
Related Products
Product Overview
All Stem Cell Differentiation Reporters are available as
lentivector plasmids or as pre-packaged lentiviral preparations (using the HIV lentiviral backbone and
pseudotyped with VSV-G protein)—ready for transduction of target
cells. The standard product size is 10
mg of plasmid or 1 x 106 IFUs per packaged
lentiviral reporter. Positive and negative transduction control
plasmids and pre-packaged viral particles are also available.
Kit Components
Pre-packaged lentiviral particles of the reporter (1 x 106 IFUs)
or Reporter Plasmid (10
mg)
User Manual
Product Analysis Certification (PAC) Sheet
Shipped on dry ice, should be stored at -70°C upon receipt
Click
here to download list of reporters with associated catalog
numbers
Vector Schematic
Sample data in Embryonic Stem Cells
Sample data for Neural Differentiation Reporter
Panel A
 The GFAP gene is differentially expressed in glioma cell
lines. GFAP-positive U251 cells express GFP when transduced with
the GFAP-GFP reporter virus (Panel A). The reporter is silent in
GFAP-negative T98 cells (Panel B). Both glioma cell lines are
readily infected with a control lentivirus (CMV-GFP).
Panel B
List of Differentiation Reporters
Reporters available that enable differentiation monitoring of
several lineages in a wide variety of cell types
|
Target Cell type |
Species |
Promoter/Enhancer Element |
Official name |
Other Names |
Packaged Virus Catalog # |
Reporter plasmid Catalog # |
|
Chondrocyte |
Mouse |
Col2a1 |
Col2a1 |
collagen, type II, alpha 1 |
SR1001VA-1 |
SR1001PA-1 |
|
Osteoblast |
Human |
SPP1 |
SPP1 |
secreted phosphoprotein 1 (SPP1), osteopontin (OPN) |
SR1002VA-1 |
SR1002PA-1 |
|
Osteoblast |
Human |
Osteocalcin |
BGLAP |
bone gamma-carboxyglutamate (BGLAP), BGP, PMF1 |
SR1003VA-1 |
SR1003PA-1 |
|
B-cell |
Human |
B29 |
CD79B |
CD79b, Ig-beta, Igb, Igbeta |
SR1004VA-1 |
SR1004PA-1 |
|
B-cell |
Mouse |
B29 |
Cd79b |
Cd79b |
SR1005VA-1 |
SR1005PA-1 |
|
CD8 T-cell |
Mouse |
CD8 |
Cd8a |
BB154331, Ly-2, Ly-35, Ly-B, Lyt-2 |
SR1006VA-1 |
SR1006PA-1 |
|
Erythroid |
Human |
HLA-DRa |
HLA-DRA |
HLA-DRA1, major histocompatibility complex, class II, DR
alpha |
SR1007VA-1 |
SR1007PA-1 |
|
Macrophage, microglia |
Mouse |
Cd68 |
Cd68 |
macrosialin, Scard1, gp110 |
SR1008VA-1 |
SR1008PA-1 |
|
Pan T-cell |
Human |
CD2 |
CD2 |
SRBC, T11 |
SR1009VA-1 |
SR1009PA-1 |
|
Cardiomyocyte |
Mouse |
Actc |
Actc1 |
cardiac muscle alpha actin 1 |
SR10010VA-1 |
SR10010PA-1 |
|
Cardiomyocyte |
Human |
MLC-2v |
MYL2 |
MYL2, MLC2 |
SR10011VA-1 |
SR10011PA-1 |
|
Cardiomyocyte |
Human |
TNNT2 |
TNNT2 |
troponin T type 2 (cardiac), TnTC, cTnT |
SR10012VA-1 |
SR10012PA-1 |
|
Cardiomyocyte |
Mouse |
Tnnt2 |
Tnnt2 |
Tnt, cTnT |
SR10013VA-1 |
SR10013PA-1 |
|
Smooth muscle myocyte |
Mouse |
SM22a |
Tagln |
Transgelin (Tagln), SM22, Sm22a |
SR10014VA-1 |
SR10014PA-1 |
|
Astrocyte |
Human |
GFAP |
GFAP |
Glial fibrillary acidic protein |
SR10015VA-1 |
SR10015PA-1 |
|
Astrocyte |
Mouse |
Gfap |
Gfap |
Glial fibrillary acidic protein |
SR10016VA-1 |
SR10016PA-1 |
|
Microglia |
Human |
CD11b |
ITGAM |
integrin, alpha M, CD11B, CR3A, MAC-1, MAC1A, MGC117044,
MO1A |
SR10017VA-1 |
SR10017PA-1 |
|
Microglia |
Mouse |
EMR1 |
Emr1 |
EGF-like module containing, mucin-like, hormone
receptor-like sequence 1, EGF-TM7, F4/80 |
SR10018VA-1 |
SR10018PA-1 |
|
Microglia |
Mouse |
Iba-1 |
Aif1 |
allograft inflammatory factor 1 (Aif-1), ionized calcium
binding adapter molecule 1 (Iba-1) |
SR10019VA-1 |
SR10019PA-1 |
|
Muller glia |
Mouse |
Cd44 |
Cd44 |
HERMES, Ly-24, Pgp-1 |
SR10020VA-1 |
SR10020PA-1 |
|
Neuron |
Human |
BM88 |
Cend1 |
cell cycle exit and neuronal differentiation 1 (Cend1) |
SR10021VA-1 |
SR10021PA-1 |
|
Neuron |
Mouse |
Camk2a |
Camk2a |
CaMK II; alpha-CaMKII |
SR10022VA-1 |
SR10022PA-1 |
|
Neuron |
Mouse |
GAD67 |
Gad1 |
glutamic acid decarboxylase 1, EP10, GAD67, Gad-1 |
SR10023VA-1 |
SR10023PA-1 |
|
Neuron |
Rat |
NSE |
Eno2 |
enolase 2, gamma (Eno2), neuron-specific enolase (NSE) |
SR10024VA-1 |
SR10024PA-1 |
|
Neuron |
Mouse |
Ta1
a-tubulin |
Tuba1a |
tubulin, alpha 1A (Tuba1a), Tuba-1, Tuba1 |
SR10025VA-1 |
SR10025PA-1 |
|
Oligodendrocyte |
Mouse |
MBP |
Mbp |
myelin basic protein (Mbp), myelin deficient; shiverer |
SR10026VA-1 |
SR10026PA-1 |
|
Photoreceptor |
Human |
Opsin |
OPN1SW |
opsin 1 (cone pigments), short-wave-sensitive (color
blindness, tritan) |
SR10027VA-1 |
SR10027PA-1 |
|
Beta cell |
|
