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Small RNA Quantitation System
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Rapidly tag and convert all small RNAs into detectable cDNA
for qPCR
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Simple and robust procedure
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Powerful technology to convert enough RNA to cDNA
for up to 5,000 qPCR measurements
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Validate newly discovered MicroRNAs
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Detect and Quantitate siRNAs in knock-down experiments
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Amenable to high-throughput screening of clinical samples
including laser-captured micro dissected specimens
Product Overview
Sample Data and Technology
References
Kit Components
User Manual
Ordering Information
Related Products
Click here to
download QuantiMir Sample Data and Technology Presentation
QuantiMir RT Kit Overview
What this Kit does:
The new QuantiMir RT Kit tags and converts all small RNAs into
cDNA, ready to use as template for real-time qPCR.
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Measure and profile MicroRNA expression levels from any
tissue source
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Validate the existence of newly discovered MicroRNA species
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Measure 100 or more different MicroRNAs per QuantiMir RT
reaction
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Detect and quantify siRNAs from knockdown experiments
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Entire procedure takes <2 hours
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Kit has enough reagents for 5,000 qPCR experiments
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No
more messy Northerns or RNase protection assays! Say
good-bye to Radioactivity. |
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Stop
wasting your precious RNA on single MicroRNA
detection per RT reaction! |
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Kit Provides |
User Provides |
1.
PolyA polymerase + Buffer solutions
2. Adapter dT Primer
3. Reverse Transcriptase + Buffer solutions
4. Universal 3’ qPCR Primer
5. Positive control miR-16 Primer
6. Positive control U6 snRNA Primers |
1.SYBR green master mix
2. Forward primer specific for the miRNA or siRNA of
interest
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SBI has tested and recommends SYBR Green Master mix from three
vendors: Power SYBR Master Mix® (Cat numbers 4368577, 4367650,
4367659, 4368706, 4368702, 4368708, 4367660) from Applied
Biosystems; SYBR GreenER™ qPCR SuperMix for ABI PRISM®
instrument from Invitrogen (Cat numbers 11760-100, 11760-500,
and 11760-02K); and RT² Real-Time™ SYBR Green / ROX PCR (Cat
numbers PA-012 and PA-112) from SuperArray. For details of the
buffer recommendation and experimental conditions, please see
User Manual.
Sample Data: Profile MicroRNAs from Cancer and Normal Tissues
 Sample
Data: Use QuantiMir
to measure both siRNA and mRNA levels in a single cDNA sample
Kit
Components
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40 |
μl |
5X PolyA polymerase Buffer |
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10 |
μl |
PolyA polymerase |
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20 |
μl |
25 mM MnCl2 |
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30 |
μl |
5 mM ATP |
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10 |
μl |
Oligo dT Adaptor |
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80 |
μl |
5X Reverse Transcriptase Buffer |
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20 |
μl |
Reverse Transcriptase |
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30 |
μl |
0.1 M Dithiothreitol (DTT) |
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40 |
μl |
dNTP Mix |
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600 |
μl |
3’ Universal Reverse PCR Primer |
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50 |
μl |
5’ Human U6 Control Forward Primer (10mM) |
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50 |
μl |
5’ Human miR-16 Control Forward Primer (10mM) |
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50 |
μl |
5’ Mouse U6 Control Forward Primer (10mM) |
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1.2 |
ml |
RNase-free Water |
Kit should be stored at -200
C
Ordering Information
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Product Description |
Catalog# |
Size |
Price |
To Order |
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QuantiMir RT Kit |
RA420A-1 |
20
reactions |
$425 |
To Order |
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Cells-to-Cts plus QuantiMir RT Kit |
RA422A-1 |
20
reactions |
$450 |
To Order |
To obtain more information contact SBI at 888-266-5066
(toll-free in North America) or 650-968-2200, or e-mail to
orders@systembio.com
User
Manual for QuantiMir
Protocol for
Cells-to-Cts
Related Products
Global MicroRNA Amplification KitTM
MicroRNA DiscoveryTMKit
Cancer MicroRNA Profiling Panel
Stem Cell MicroRNA Profiling Panel
HT 384 MicroRNA Profiling
System
HT 419 MicroRNA Profiling System
Technical References
Shi, R. and Chiang, V.L. 2005. Facile means for quantifying
microRNA expression by real-time PCR. BioTechniques. 39:519-525.
Fortner, D.M., Troy, R.G., and Brow D.A. 1994. A stem/loop in U6
RNA defines a conformational switch required for pre-mRNA
splicing. Genes and Development. 8(2): 221-233.
Cancer and MicroRNA References
Click here for Reference list with PubMed
links
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