• Choice of promoters with different strength and constitutive expression in various cell types

• HIV or FIV backbone available

• GFP or puromycin resistance reporter gene for easy selection or screening

• Compatible with SBI’s lentiviral packaging system for efficient delivery and stable expression in target cells

• Click here to download vector maps
  
   

cDNA Cloning and Expression Vectors

• New HIV-based single promoter vectors with dual expression cassette based on T2A peptide

• Housekeeping elongation factor 1 α promoter (EF-1α) provides robust expression in most cell types

• CpG-deficient MSCV promoter for efficient expression in embryonic stem cells and hematopoietic cells
   

EF1: Elongation factor 1X promoter
MSCV: Murine stem cell virus LTR enhancer/promoter
CMV: Cytomegalovirus (CMV) promoter
MCS: Multicloning sites
T2A: 2A peptide from Thosea asigna (italian)

Fig. 1. Flow cytometry analysis of HT1080 cells transduced with dual reporter constructs. The puromycin-resistance gene (puro) was cloned into pCDH2-EF1-MCS3-T2A-copGFP (A) and pCDH2-MCS4-T2A-copGFP-MSCV (B); and the copGFP gene (cG) was cloned into pCDH2-EF1-MCS3-T2A-Puro (C) and pCDH2-MCS4-T2A-Puro-MSCV (D). The resulting dual reporter constructs were packaged into pseudoviral particles followed by transduction into HT1080 cells. All constructs were also puromycin resistant (data not shown).

User Manual

shRNA Cloning and Expression Vectors

• Choose the best internal promoter for stable expression of GFP or puromycin reporter gene in your target cells

• New cloning sites (two Bbs I) for single digestion and directional cloning with decreased background

• shRNA expression under either H1 or U6 promoter for maximum knock down effect in different cell types

Packaged Transduction Control New Sampler Pack!