PEG-it™ Virus Precipitation Solution

Easily Concentrate Lenti- and Retroviruses with PEG-it™ Virus Precipitation Solution

• Easy to use and cost-effective method for highly efficient viral concentration

• Concentrate all lentiviral and retroviral particles 10- to 100-fold

• Non-toxic reagents safe for use with all target cell lines tested, including ES cells (see Sample Data below)

• No special equipment required - avoid the expense of an ultracentrifuge

• No accumulation of cellular debris like with ultracentrifugation - avoid toxic and immunogenic reactions by using PEG-it™ instead

• Ideally suited for concentrating virus from large volumes - avoid the limited capacity per spin of ultracentrifugation

• Improve transduction of cells and prepare ultra-high titer preps for in vivo infections

• Sterile and ready for use in cell culture

• Completely compatible with System Bioscience’s LentiMag™ Magnetotransduction Kit

PEG-it™ Virus Precipitation Solution provides a simple and highly effective means to concentrate lentiviral and retroviral particles, such as those produced with System Biosciences' pPACK Lentivector Packaging System.  PEG-it™ Virus Precipitation Solution is a formulation of polyethylene glycol optimized for the precipitation of all retro and lentiviral-based particles.  The solution is mixed with virus-containing cell culture supernatant, incubated at 4°C overnight, and centrifuged at 1500 × g to pellet precipitated viral particles.  As a result, viral particle concentrations are increased 10- to 100-fold (Fig. 1).

Sample Data of PEG-it™ Precipitated Virus Infecting hES Cells

Ordering Information

* - 100 ml of sterile PEG-it™ reagent is sufficient for precipitation of lentiviral particles from 400 ml of cell culture supernatant.
 

** - 250 ml of sterile PEG-it™ reagent is sufficient for precipitation of lentiviral particles from 1L of cell culture supernatant.

User Manual