Lentiviral Magneto Transduction Kit

Increase Transduction Efficiency with LentiMag™

  • Increase percentage of cells transduced with System Bioscience’s lentiviral vector compared to transductions performed alone or with polybrene

  • Concentrate lentiviral vector dose onto cells very rapidly

  • Successfully transduce nonpermissive (difficult-to-infect) cells and cell lines

  • Compatible with adherent cells and cells grown in suspension

  • Significantly improve transduction with low titer lentiviral vector doses

  • Economical, simple to use, and rapid

  • Nontoxic; does not require the use of polybrene

  • Sterile; ready for use in cell culture

  • Can be used with both Lentiviruses & retroviruses from a number of sources

The LentiMag™ kit provides a novel, simple, and highly effective approach to increase the number of cells positively transduced with System Bioscience’s HIV- and FIV-based lentiviral vectors compared to transduction aided by addition of polybrene. LentiMag™ is a nanoparticle formulation specifically developed for transduction of mammalian cells using Retrovirus- or Lentivirus-based gene and siRNA delivery vectors. Lentiviral vectors are complexed with magnetic nanoparticles, which are then placed within a strong magnetic field to rapidly pull the virus particles towards the target cells. In this manner, the applied viral dose is concentrated onto cells within minutes so that most of the cells come into contact with the lentiviral vector inoculum (Fig. 1). As a result, the number of transduced cells is increased by up to 3-fold as compared to traditional transductions performed using polybrene (Fig. 2 a&b).

LentiMag™ reagent requires a strong magnetic field to enhance transduction of target cells with lentiviral vectors. The magnetic plate supplied with the kit has been specifically designed to exert this magnetic field. The geometry of the plate is compatible with all standard cell culture dishes, including T-25 and T-75 flasks, 60 and 100 mm diam. dishes, and 6-, 12-, 24-, 48-, & 96-well plates.

 

SBI LentiMag™ kit:
Cat. # LV800A-1        Price: $490   To Order
Includes 50 μl sterile LentiMag™ reagent (10 transductions in a 24-well plate format) and one magnetic plate.

LentiMag™ Reagent:    
Cat. # LV801A-1        Price: $300   To Order
LentiMag™ Reagent only, for customers who have already purchased magnetic plate in above kit. Sterile, 50 μl  (10 transductions in a 24-well plate format).
 

PDF Manual

 

 

Fig. 1

 

Fig. 2a

 

Fig 2b