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Choose
the type of Lentivector based upon what you want to express:
Click
on vector image for more details
The easiest method for efficient & accurate cloning of cDNAs, shRNAs or
microRNA precursors into Lentivectors with ready-to-use Heat'n'Mix
cloning systems. Lentiviral delivery provides for easy and
efficient transduction into a wide-range of dividing and non-dividing
cell types, including primary and stem cells.
Comparison between
Heat'n'Mix and Standard Cloning Vectors:
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Heat’n’Mix system |
Ligase-dependent method |
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Digestion of PCR products and vectors |
No |
Yes |
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Gel purification of digests |
No |
Yes |
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CIP vector to reduce background |
No |
Yes |
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Ligation |
30 min of Heat’n’Mix |
1 hour to overnight |
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Hands-on time |
About 10 min |
1 to 2 hours |
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Efficiency |
> 90% |
Low |
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Fidelity |
High |
Medium |
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Sequence compatibility |
No limit |
Limited by restriction sites |
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Primer design |
Simple |
Complicated |
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Built-in in-frame indicator for cDNA
cloning |
Yes |
No |
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Cost effective |
Yes ($25, 4 oligos/shRNA) |
No ($65, 2 oligos/shRNA) |
User Manual
PDF User Manual
Procedural Overview:
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