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Features and Benefits
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Real-time qPCR-based microRNA expression profiling system
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Profile 419 different MicroRNAs known to be involved in all
facets of cellular regulation
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SBI’s approach rapidly tags and converts all small RNAs into
detectable cDNAs for qPCR – one cDNA synthesis required to
quantitate our entire 419 microRNA assay set
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Validate newly discovered MicroRNAs
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Sensitive and quantitative down to picogram amounts of
starting total RNA, start with 400 ng to profile 419
microRNAs
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Human or Mouse U6 control included in panels
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Amenable to high-throughput analysis of numerous cell lines,
tissues and clinical samples
Click here to download
and view HT 419 microRNA Profiler Array Layout
Product Overview
References
Kit Components
User Manual
Ordering Information
Related Products
Click here to
download QuantiMir Sample Data and Technology Presentation
HT 419 microRNA Profiler Overview
What this Kit does:
The new HT 419 microRNA Profiler enables the quantitation of 419
different microRNAs along with a control for U6 for
normalization.
SBI’s technology utilizes the same approach as in the QuantiMir
RT Kit which tags and converts all small RNAs into cDNAs, ready
to use as template for real-time qPCR.
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Quantitate and profile MicroRNA expression levels from any
cell type or tissue source
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Discover microRNA Biomarkers in Cancer & Stem Cell
development
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Identify expression pattern signatures for Cell-specific
lineages and responses to external stimuli
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Sensitive SYBR green-based detection system
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Profile 419 different microRNAs from 1 cDNA
synthesis using as little as 400 ng of total RNA |
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Kit Provides |
User Provides |
1.
PolyA polymerase + Buffer solutions
2. Adapter dT Primer
3. Reverse Transcriptase + Buffer solutions
4. Universal 3’ qPCR Primer
5. Primer set for 419 MicroRNAs plus U6 on each of 5
x 96-well plates
6. Complete kit for 10 entire profiles
7. CD with MicroRNA ID lists and simple relative
quantitation spreadsheet calculator |
1. RNA samples
2. SYBR green master mix
3. Optical qPCR plates
4. Real-time qPCR instrument
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SBI has tested and recommends SYBR Green Master mix from three
vendors: Power SYBR Master Mix® (Cat numbers 4368577, 4367650,
4367659, 4368706, 4368702, 4368708, 4367660) from Applied
Biosystems; SYBR GreenER™ qPCR SuperMix for ABI PRISM®
instrument from Invitrogen (Cat numbers 11760-100, 11760-500,
and 11760-02K); and RT² Real-Time™ SYBR Green / ROX PCR (Cat
numbers PA-012 and PA-112) from SuperArray. For details of the
buffer recommendation and experimental conditions, please see
User Manual.
Technical References
Shi, R. and Chiang, V.L. 2005. Facile means for quantifying
microRNA expression by real-time PCR. BioTechniques. 39:519-525.
Fortner, D.M., Troy, R.G., and Brow D.A. 1994. A stem/loop in U6
RNA defines a conformational switch required for pre-mRNA
splicing. Genes and Development. 8(2): 221-233.
Cancer and MicroRNA References
Click here for Reference list with PubMed
links
Kit
Components
HT 419 assay set comes in 5 x 96-well microRNA Primer stock
plates (enough for 10 entire panel measurements)
Kits should be stored at -200
C upon receipt.
User
Manual
Ordering Info
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Product Description |
Catalog# |
Size |
Price |
To Order |
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HT 419 microRNA Profiler |
RA630A-1 |
10
complete profiles |
$1,295 |
To Order |
To obtain more information contact SBI at 888-266-5066
(toll-free in North America) or 650-968-2200, or e-mail to
orders@systembio.com
Related Products
QuantiMir™
Small RNA Quantitation System
Global MicroRNA
Amplification KitTM
MicroRNA DiscoveryTMKit
HT 384 MicroRNA Profiling
System
Cancer MicroRNA qPCR
Array
Stem Cells MicroRNA pPCR Array
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