Features and Benefits: 

• Real-time qPCR-based microRNA expression profiling system

• Simultaneously profile 383 different MicroRNAs known to be involved in all facets of cellular regulation.

• SBI’s approach rapidly tags and converts all small RNAs into detectable cDNAs for qPCR – one cDNA synthesis required to quantitate our entire 384-well plate

• Sensitive and quantitative down to picogram amounts of starting total RNA, start with 400 ng to profile 383 microRNAs

• Human or Mouse U6 control included in panel

• Amenable to high-throughput analysis of numerous cell lines and tissues

Click here to download and view HT 384 microRNA Profiler Array Layout

Product Overview
Technical_Reference
Kit Components
User Manual
Ordering Information
Related Products

HT 384 microRNA Profiler Overview

What this Array Kit does:

The new HT 384 microRNA Profiler enables the simultaneous quantitation of 383 microRNAs along with a control for U6.

SBI’s technology utilizes the same approach as in the QuantiMir RT Kit which tags and converts all small RNAs into cDNAs, ready to use as template for real-time qPCR.

• Quantitate and profile MicroRNA expression levels from any cell type or tissue source

• Discover microRNA Biomarkers in Cancer & Stem Cell development

• Identify expression pattern signatures for Cell-specific lineages and responses to external stimuli

• Sensitive SYBR green-based detection system

• Optimized for efficient 5 μl qPCR reaction volumes
  

Profile 383 different microRNAs simultaneously from 1 cDNA synthesis using as little as 400 ng of total RNA

SBI has tested and recommends SYBR Green Master mix from three vendors: Power SYBR Master Mix® (Cat numbers 4368577, 4367650, 4367659, 4368706, 4368702, 4368708, 4367660) from Applied Biosystems; SYBR GreenER™ qPCR SuperMix for ABI PRISM® instrument from Invitrogen (Cat numbers 11760-100, 11760-500, and 11760-02K); and RT² Real-Time™ SYBR Green / ROX PCR (Cat numbers PA-012 and PA-112) from SuperArray. For details of the buffer recommendation and experimental conditions, please see User Manual.

Technical Reference

Shi, R. and Chiang, V.L. 2005. Facile means for quantifying microRNA expression by real-time PCR. BioTechniques. 39:519-525.

Fortner, D.M., Troy, R.G., and Brow D.A. 1994. A stem/loop in U6 RNA defines a conformational switch required for pre-mRNA splicing. Genes and Development. 8(2): 221-233.

Kit Components

HT 384-well microRNA Primer stock plate comes with assays dried down in wells. Resuspend each well in 10ml water before use (enough for 10 entire panel measurements).

Kits should be stored at -20^oC upon receipt.

User Manual

Ordering Information

To obtain more information contact SBI at 888-266-5066 (toll-free in North America) or 650-968-2200, or e-mail to info@systembio.com

Related Products

QuantiMir^™ Small RNA Quantitation System
Cancer MicroRNA  qPCR Array
Stem Cell MicroRNA qPCR Array
Global MicroRNA Amplification Kit^™
MicroRNA Discovery^™ Kit