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Stably
express double-stranded siRNA molecules
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Clone short,
stable oligonucleotide templates
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Construct complex
siRNA libraries using short double-stranded DNA templates
SBI’s
double-promoter pFIV-H1/U6 siRNA Cloning and Expression Vectors
allows you to clone and express natural double-stranded
siRNA constructs, as opposed to hairpin-type
siRNA (also known as shRNA) constructs. Short double-stranded siRNA template
oligonucleotides are less expensive and more stable during propagation than
hairpin shRNA templates.
In the pFIV-H1/U6 siRNA Vectors, the expression cassette is flanked by
opposing H1 and U6 RNA Polymerase III modified promoters and T5 terminator
sequences. Successfully cloned siRNA template oligonucleotides are
transcribed in both the forward and reverse directions, by the opposing
promoters, to express double-stranded siRNA—similar to the structure of
naturally occurring siRNA molecules.
After transduction, positive clones that are stably expressing the siRNA can
be isolated by sorting with a flow cytometer (when using pFIV-H1/U6-copGFP)
or selecting for puromycin resistance (for pFIV-H1/U6-Puro).
Double-Promoter siRNA Vector Products
FIV-Based siRNA Vectors
pFIV-H1/U6-Puro siRNA Cloning and Expression Vector
pFIV-H1/U6-copGFP siRNA Cloning and Expression Vector
Related
Products
Single Promoter shRNA Cloning and Expression Lentivectors
pPACK Lentivector Packaging System
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