High-throughput MicroRNA
Profiling Using Powerful and Sensitive Real-time qPCR
Features and Benefits
-
Simultaneously profile 95 different MicroRNAs known to be
involved in apoptosis, differentiation and Cancer
-
Identify MicroRNA biomarkers and expression pattern
signatures
-
System rapidly tags and converts all small RNAs into
detectable cDNA for qPCR
-
Simple and robust procedure
-
Sensitive and quantitative down to picogram amounts of
starting total RNA
-
Amenable to high-throughput screening of clinical samples
including biopsies and LCM samples
Click here to download
Cancer MicroRNA qPCR Array Technology Overview presentation
Product Overview
Sample Data and
Technology Overview
References
Kit Components
User Manual
Ordering Information
Related Products
Cancer
MicroRNA qPCR Array Overview
What this Array Kit does:
The new Cancer MicroRNA qPCR Array enables the simultaneous
quantitation of 95 MicroRNAs known to be involved in Cancer.
The technology utilizes the QuantiMir RT Kit which tags and
converts all small RNAs into cDNA, ready to use as template for
real-time qPCR.
-
Quantitate and profile MicroRNA expression levels from any
tissue source
-
Discover Biomarkers in your Cancer samples
-
Develop expression pattern signatures in Cancer LCM samples
-
Measure 40 or more different MicroRNAs per QuantiMir RT
reaction
-
Entire procedure takes 1 day.
No more messy
Northerns or RNase protection assays! Say good-bye to
Radioactivity.
Stop wasting
your precious RNA on a single MicroRNA detection per RT
reaction!
Profile 95 different MicroRNAs
simultaneously from 1 RT reaction!
| Kit
Provides |
User Provides |
1.
PolyA polymerase + Buffer solutions
2. Adapter dT Primer
3. Reverse Transcriptase + Buffer solutions
4. Universal 3’ qPCR Primer
5. Primer set for 95 MicroRNAs for 10 qPCR plates
6. Positive control U6 snRNA Primer
7. CD with MicroRNA ID lists and simple relative
quantitation spreadsheet calculator |
1. RNA
samples
2. SYBR green master mix
3. Optical qPCR plates
4. Real-time qPCR instrument
|
SBI has tested and recommends SYBR Green Master mix from three
vendors: Power SYBR Master Mix® (Cat numbers 4368577, 4367650,
4367659, 4368706, 4368702, 4368708, 4367660) from Applied
Biosystems; SYBR GreenER™ qPCR SuperMix for ABI PRISM®
instrument from Invitrogen (Cat numbers 11760-100, 11760-500,
and 11760-02K); and RT² Real-Time™ SYBR Green / ROX PCR (Cat
numbers PA-012 and PA-112) from SuperArray. For details of the
buffer recommendation and experimental conditions, please see
User Manual.
Cancer MicroRNA qPCR Array
Layout
click to enlarge
Application: Profile
MicroRNAs from Cancer and Normal Tissues
A susbset of the MicroRNA primers were used in this study to
confirm published findings of 9 different MicroRNAs in 5
separate tumor vs. Normal RNA samples.
Sample MicroRNA qPCR Array Data:
User-generated qPCR Data from Human clinical biosies from Normal
and Tumor tissue.
Technical References
Shi, R. and Chiang, V.L. 2005. Facile means for quantifying
microRNA expression by real-time PCR. BioTechniques. 39:519-525.
Fortner, D.M., Troy, R.G., and Brow D.A. 1994. A stem/loop in U6
RNA defines a conformational switch required for pre-mRNA
splicing. Genes and Development. 8(2): 221-233.
Cancer and MicroRNA
References
Click here for Reference
list with PubMed links
Kit Components
Kits should be stored at -20oC upon receipt.
User Manual
Ordering Information
|
Product Description |
Catalog# |
Size |
Price |
To Order |
|
Cancer
microRNA
qPCR
Array |
RA610A-1 |
10
complete profiles |
$795 |
To Order |
To obtain more information contact SBI at 888-266-5066
(toll-free in North America) or 650-968-2200, or e-mail to
orders@systembio.com
Related Products
QuantiMir™
Small RNA Quantitation System
Global MicroRNA Amplification Kit™
MicroRNA Discovery™
Kit
Stem Cell MicroRNA Profiling Panel
HT 384 MicroRNA Profiling
System
HT 419 MicroRNA Profiling System |
